Results
| PMID | 25366587 |
| Gene Name | PLA2G2A |
| Condition | Endometriosis (ovarian) |
| Association |
Associated |
| Population size | 116 |
| Population details | 116 (70 ovarian endometriosis, 38 healthy women controls, 8 patients with benign ovarian cysts) |
| Age | Patients: 32.9 ± 6.2 yrs, control women- 37.1 ± 9.8, 39.5 ± 3.8) |
| Sex | Female |
| Other associated phenotypes |
Ovarian endometriosis |
|
Gynecol Endocrinol. 2015 Mar;31(3):214-8. doi: 10.3109/09513590.2014.975690. Epub Kocbek, Vida| Bersinger, Nick A| Brglez, Vesna| Mueller, Michael D| Petan, Toni| Rizner, Tea Lanisnik Institute of Biochemistry, Faculty of Medicine, University of Ljubljana , Ljubljana , Slovenia . Our previous gene expression analysis identified phospholipase A2 group IIA (PLA2G2A) as a potential biomarker of ovarian endometriosis. The aim of this study was to evaluate PLA2G2A mRNA and protein levels in tissue samples (endometriomas and normal endometrium) and in serum and peritoneal fluid of ovarian endometriosis patients and control women. One-hundred and sixteen women were included in this study: the case group included 70 ovarian endometriosis patients, and the control group included 38 healthy women and 8 patients with benign ovarian cysts. We observed 41.6-fold greater PLA2G2A mRNA levels in endometrioma tissue, compared to normal endometrium tissue. Using Western blotting, PLA2G2A was detected in all samples of endometriomas, but not in normal endometrium, and immunohistochemistry showed PLA2G2A-specific staining in epithelial cells of endometrioma paraffin sections. However, there were no significant differences in PLA2G2A levels between cases and controls according to ELISA of peritoneal fluid (6.0 +/- 4.4 ng/ml, 6.6 +/- 4.3 ng/ml; p = 0.5240) and serum (2.9 +/- 2.1 ng/ml, 3.1 +/- 2.2 ng/ml; p = 0.7989). Our data indicate that PLA2G2A is implicated in the pathophysiology of ovarian endometriosis, but that it cannot be used as a diagnostic biomarker. Mesh Terms: Adult| Ascitic Fluid/*metabolism| Case-Control Studies| Endometriosis/blood/genetics/*metabolism| Endometrium/*metabolism| Female| Group II Phospholipases A2/blood/genetics/*metabolism| Humans| Middle Aged| Ovarian Cysts/blood/genetics/metabolism |
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